Antibiotic optimization via in vitro glycorandomization
- 9 November 2003
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 21 (12), 1467-1469
- https://doi.org/10.1038/nbt909
Abstract
In nature, the attachment of sugars to small molecules is often used to mediate targeting, mechanism of action and/or pharmacology. As an alternative to pathway engineering or total synthesis, we report a useful method, in vitro glycorandomization (IVG), to diversify the glycosylation patterns of complex natural products. We have used flexible glycosyltransferases on nucleotide diphosphosugar (NDP-sugar) libraries to generate glycorandomized natural products and then applied chemoselective ligation to produce monoglycosylated vancomycins that rival vancomycin.Keywords
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