Long-Term Cultivation in Tissue Culture of Leukemic Cells From Mouse Leukemia Induced by Moloney Virus or by X Rays2

Abstract

Successful long-term in vitro cultures of leukemic cells have been obtained from BALB/c, C57BL/6, SWR, and Swiss mice, with leukemia induced by Moloney virus; and from C57BL/6 mice, with leukemia induced by X rays. The leukemic cells were grown from 4 to 10 months in vitro. All the cell lines were successfully maintained on feeder layers of normal mouse spleen cells in Eagle's medium supplemented with horse serum. Under these conditions the leukemic cells grew free in the medium, so that they could be readily passaged without trypsinization. The leukemic nature of the cultured cells was established by examination of cell smears and by cell transplantation into animals.