Gas Chromatographic Study of Phosphatidyl Serines

Abstract

The reaction of silylating agents (BSA-TMCS) and phosphatidyl serines leads to the formation of 1,2-diglyceride trimethylsilyl (TMSi) ethers, along with small amounts of related 1,3-diglyceride trimethylsilyl ethers. The major product from a bovine phosphatidyl serine fraction was found to be 1-stearoyl-2-oleoylglycerol TMSi ether (the isomeric structure 1-oleoyl-2-stearoylglycerol TMSi ether may also be present). Related diglycerides were also found as products. The reaction products were identified by gas chromatography-mass spectrometry. The mechanism of the reaction is unknown, but it provides a way of studying the composition of phosphatidyl serine fractions without prior enzymic or chemical hydrolysis.