Safranin and Anilin Blue with Delafield's Hematoxylin for Staining Cell Walls in Shoot Apexes
- 1 January 1948
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 23 (4), 185-190
- https://doi.org/10.3109/10520294809106246
Abstract
The following technic is suggested for staining cell walls in shoot apexes: After the usual preliminary steps through 50% ethyl alcohol, stain in 1 % safranin 0 for 24 hours. Rinse in tap water and place in 2% aqueous tannic acid for 2 minutes. After rinsing in tap water, stain for 2 minutes in 1 part Delafield's hematoxylin to 2 parts distilled water and rinse in tap water. Remove excess hematoxylin with acidified water (1 drop cone. HC1 in 200 ml. water), then place slides in 0.5% lithium carbonate for 5 minutes. Dehydrate through an ethyl alcohol series, then transfer from absolute alcohol to a saturated solution of anilin blue in “methyl cellosolve” for 5-10 minutes. Wash in absolute alcohol, rinse in a solution of 25% methyl salicylate, 33% xylene, 42% absolute ethyl alcohol and clear for 10 minutes in a solution of 2 parts methyl salicylate, 1 part xylene, 1 part absolute ethyl alcohol. Transfer through two changes of xylene and mount in “clarite” or suitable alternate. The resulting preparations will have clearly defined, dark-staining cell walls and will photograph well when “Super Panchro-Press, Type B” film (Eastman Kodak Co.) is used in conjunction with suitable Wratten filters.Keywords
This publication has 2 references indexed in Scilit:
- Delafield's Hematoxylin and Safranin for Staining Meristematic TissuesStain Technology, 1939
- The Use of Tannic Acid and Iron Chloride for Staining Cell Walls in Meristematic TissueStain Technology, 1934