Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.
- 1 July 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (7), 2191-2195
- https://doi.org/10.1073/pnas.73.7.2191
Abstract
The synthesis of large, possibly complete, complementary DNA (cDNA) copies of poliovirus RNA by avian myeloblastosis virus DNA polymerase is described. The cDNA consists of 2 size classes, the larger of which is approximately 7500 nucleotides. In the presence of excess deoxynucleoside triphosphates, ribonucleoside triphosphates or sodium pyrophosphate, only the larger material is obtained. Yields of the large cDNA are 50-75% of the input RNA.This publication has 26 references indexed in Scilit:
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