New fluorescent cytidine 5'-phosphate derivatives
Open Access
- 1 May 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (5), 1341-1350
- https://doi.org/10.1093/nar/3.5.1341
Abstract
Interaction of cytidine 5′phosphate with chloroacetone or p-tosyloxyacetone leads to 2-methyl-5,6-dihydro-5-oxo-6-(5-O-pospho-β-D-ribofuranosyl)-imidazo/1,2-c/pyrimidine (2-methyl-ethenocytidine 5′-phosphate) whereas analogous reaction with phenacyl bromide produces similar 2-phenyl-derivative. The bicyclic nucleotides obtained showed significant UV absorption at long wavelength where common nucleotides and proteins exhibited no absorption. These derivatives are highly fluorescent when heterocyclic ring is protonated. The absorption and fluorescent properties of the substituted ethenocytidine 5′-phosphate derivatives seem to be suitable for their use as fluorescent probes or labels in biochemical studies.Keywords
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