The angiogenesis inhibitor endostatin impairs blood vessel maturation during wound healing

Abstract

SPECIFIC AIMSIn this study we determined the effect of the tumor angiogenesis inhibitor endostatin on the healing of full-thickness excisional wounds in mice. We studied the overall wound-healing process, the density and morphological appearance of the wound vessels, and the expression of angiogenesis regulators and of major wound matrix proteins.PRINCIPAL FINDINGS1. Endostatin does not affect wound closure, wound contraction, and wound reepithelialization, but reduces granulation tissue formationRecombinant mouse endostatin was purified to homogeneity, and a dose of 0.3 mg/(kg · day) was injected subcutaneously in the belly. Treatment was started 2 days before wounding and continued during the wound-healing process. The wounded tissue was analyzed at day 5 (15 endostatin-treated mice and 15 control mice) and day 7 (5 endostatin-treated mice and 5 control mice).No obvious abnormalities in wound closure or wound appearance were observed in the endostatin-treated mice. Histological analysis of wound section...