APPEARANCE OF SULFHYDRYL GROUPS DURING GROWTH OF BACILLUS GLOBIGII

Abstract

Good yields of the orange mutant of B. globigii are obtained when peptone, treated with norite, is included in the medium. The sulfhydryl content of spores of B. globigii is about 20% of that of the cells during the log phase. The total increase in sulfhydryl content from about 0.03 to 0.15% occurs during the late lag and early log phases of growth. The sulfhydryl content during the remaining log phase remains constant with a slow decline occurring after the termination of the log phase. When the inoculum of the medium has a high level of sulfhydryl groups, there is an initial drop followed by the increase already mentioned. Denaturation of the solubilized protein with alcohol, guanidine HCl,or urea does not increase the sulfhydryl titer.