The Relation of Immunoglobulin Class, Pattern of Antinuclear Antibody, and Complement-Fixing Antibodies to DNA in Sera from Patients with Systemic Lupus Erythematosus*
Open Access
- 1 November 1967
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 46 (11), 1785-1794
- https://doi.org/10.1172/jci105669
Abstract
Sera from 55 patients with systemic lupus erythematosus were studied to clarify the significance of the patterns of nuclear fluorescence observed. The sera in which the IgG fraction produced a peripheral pattern of nuclear fluorescence were found to contain complement-fixing antibodies to native DNA and to DNA-histone complexes. This correlation did not exist when complement-fixing activity was compared to the IgM nuclear patterns. Sera which contained only complement-fixing antibodies to denatured DNA and which did not react with native DNA or nucleoprotein did not produce the peripheral pattern of nuclear fluorescence. The data suggest that single strands of DNA were not the reactive groups in the nucleus responsible for the peripheral pattern. The results support the conclusion that DNA within a DNA-protein complex may be the nuclear antigen responsible for the peripheral pattern of nuclear fluorescence. Analysis of the clinical data revealed that a close correlation existed between the presence of IgG peripheral pattern, complement-fixing antibodies to DNA and histone-DNA complexes, and clinical manifestation of active disease.This publication has 43 references indexed in Scilit:
- Deoxybonucleic acid (DNA) and antibodies to DNA in the serum of patients with systemic lupus erythematosus.JCI Insight, 1966
- DNA haemagglutination test in the diagnosis of systemic lupus erythematosus.Annals Of The Rheumatic Diseases, 1965
- Significance of antibody to DNA in systemic lupus erythematosusArthritis & Rheumatism, 1964
- Gamma2, Gamma1A, and Gamma1M Antinuclear Factors in Human Sera*JCI Insight, 1964
- Antibodies to denatured deoxyribonucleic acid in lupus erythematosus serum II. Characterization of antibodies in several seraBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1962
- CORRELATION OF ANTINUCLEAR ANTIBODIES AND NUCLEAR STAINING PATTERNSThe Lancet, 1961
- VARIATIONS IN THE MORPHOLOGICAL PATTERNS OF "AUTOIMMUNE" NUCLEAR FLUORESCENCEThe Lancet, 1961
- The role of antinuclear reactions in the diagnosis of systemic lupus erythematosus: A study of 53 casesArthritis & Rheumatism, 1961
- CHROMATOGRAPHIC STUDIES OF THE RHEUMATOID FACTORThe Journal of Experimental Medicine, 1959
- Leuco‐précipitines II, Mise en évidence d'une réaction de précipitation entre des extraits leucocytaires et le sérum de malades atteints de lupus érythémateux disséminé*Vox Sanguinis, 1957