Stoichiometry of GTP hydrolysis in a poly(U)‐dependent cell‐free translation system
- 10 December 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 178 (2), 283-287
- https://doi.org/10.1016/0014-5793(84)80617-1
Abstract
The Stoichiometry of GTP hydrolysis during peptide elongation in the processes of codon‐specific translation and misreading of polyuridylic acid was determined in a cell‐free system in which all ribosomes were active in peptide synthesis. Ribosomes carrying oligophenylalanine presynthesized on poly(U) covalently bound to Sepharose were used. In the codon‐specific translation of poly(Phe) on poly(U)‐Sepharose at optimal Mg2+ concentration (6 mM MgCl2), the ratio of GTP cleaved to Phe polymerized was found to be about 2 (+‐ 0.1). Under the same conditions but during misreading (elongation of polyleucine on poly(U)‐Sepharose) the GTP/Leu ratio increased 10 times (from 16 to 25 in different experiments).Keywords
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