Partial purification of two lithocholic acid-binding proteins from rat liver 100000g supernatants
- 1 September 1977
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 165 (3), 425-429
- https://doi.org/10.1042/bj1650425
Abstract
The partial purification of 2 lithocholic acid-binding proteins from liver 100,000 g supernatants is described. Gel-filtration, (NH4)2SO4 fractionation. Ca3(PO4)2 fractionation and ion-exchange chromatography were used. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.This publication has 12 references indexed in Scilit:
- Binding of bile acids by 100 000g supernatants from rat liverBiochemical Journal, 1977
- The Non‐Covalent Binding of Small Molecules by LigandinEuropean Journal of Biochemistry, 1976
- Equilibrium-dialysis studies of the interaction between cholic acid and 100000g-supernatant preparations from the rat liverBiochemical Journal, 1976
- Uptake of Taurocholic Acid into Isolated Rat‐Liver CellsEuropean Journal of Biochemistry, 1975
- Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.1974
- The Identity of Glutathione S -Transferase B with Ligandin, a Major Binding Protein of LiverProceedings of the National Academy of Sciences, 1974
- ROLE OF HEPATIC ANION-BINDING PROTEIN IN BROMSULPHTHALEIN CONJUGATIONThe Journal of Experimental Medicine, 1973
- Ligandin: a Hepatic Protein which Binds Steroids, Bilirubin, Carcinogens and a Number of Exogenous Organic AnionsNature, 1971
- Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anionsJournal of Clinical Investigation, 1969
- Factors determining the maximal rate of organic anion secretion by the liver and further evidence on the hepatic site of action of the hormone secretinThe Journal of Physiology, 1966