A Nucleic Acid Associated with a Killer Strain of Yeast
Open Access
- 1 April 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (4), 1069-1072
- https://doi.org/10.1073/pnas.70.4.1069
Abstract
A crude membrane fraction was isolated from both killer M(k) and isogenic nonkiller M(o) strains of yeast labeled with [(3)H]adenine. Nucleic acids were extracted from this fraction and centrifuged to equilibrium in an ethidium bromide-Cs(2)SO(4) solution. A peak of radioactivity coincident with a single fluorescent band was present in the membrane fraction isolated from cells of the killer strain but was absent from that isolated from nonkiller cells. This material has been characterized as double-stranded RNA by treatment with various nucleases and by chromatography on a cellulose CF-11 column. Sedimentation in a sucrose gradient indicates an S value of 8-10.Keywords
This publication has 11 references indexed in Scilit:
- Curing of a Killer Factor in Saccharomyces cerevisiaeProceedings of the National Academy of Sciences, 1972
- A New Species of Double-stranded RNA from YeastNature, 1972
- Mating Pairs of Saccharomyces cerevisiae infected with Double Stranded RNA Viruses from Aspergillus nigerNature New Biology, 1972
- Electron Microscopy of Cells of Saccharomyces cerevisiae infected with Double Stranded RNA Viruses from Aspergillus niger and Penicillium stoloniferumNature New Biology, 1972
- Isolation of Circular DNA from a Mitochondrial Fraction from YeastProceedings of the National Academy of Sciences, 1972
- Penicillium cyaneo-fulvum Virus and Interferon StimulationNature, 1969
- Viruses in Fungi and Interferon StimulationNature, 1968
- Purification and Properties of Ribonuclease III from Escherichia coliJournal of Biological Chemistry, 1968
- Studies on reovirus RNAJournal of Molecular Biology, 1967
- Studies on reovirus RNA: I. Characterization of reovirus genome RNAJournal of Molecular Biology, 1967