An NADP-dependent Glutamate Dehydrogenase in Chloroplasts from the Marine Green Alga Caulerpa simpliciuscula

Abstract
NADP-dependent glutamate dehydrogenase was partially purified from extracts of the marine siphonous green alga C. simpliciuscula. The enzyme had an apparent Km NH4+ of 0.4-0.7 mM and was highly specific for NADPH, .alpha.-ketoglutarate, and ammonium ions. The bulk of the NADP-glutamate dehydrogenase was isolated with the chloroplast fraction in cell-free preparations of this alga and was released from these "chloroplast fractions" as a soluble enzyme on gentle lysis of chloroplast membranes.