Despite the popularity of digital imaging devices (e.g., CCD array cameras) the problem of accurately characterizing the spatial frequency response of such systems has been largely neglected in the literature. This paper describes a simple method for accurately estimating the optical transfer function of digital image acquisition devices. The method is based on the traditional knife-edge technique but explicitly deals with fundamental sampled system considerations: insufficient and anisotropic sampling. Results for both simulated and real imaging systems demonstrate the accuracy of the method.