p‐Hyroxybenzoate Hydroxylase from Pseudomonas fluorescens
- 1 June 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 133 (1), 91-108
- https://doi.org/10.1111/j.1432-1033.1983.tb07433.x
Abstract
As a final step in the elucidation of the primary structure of p-hydroxybenzoate hydroxylase from P. fluorescens, the amino acid sequences of a CNBr peptide (CB1, positions 111-276), that accounts for the middle part of the sequence, and the C-terminal CNBr peptide (CB2, positions 277-394) from the enzyme were determined. Important sequence information was obtained from 2 subfragments that were formed by the cleavage with CNBr of the Met-Thr sequence (positions 346-347) in peptide CB2. The alignment of the 2 subfragments from peptide CB2 and three 1-residue overlaps between peptides from one of these subfragments were confirmed by investigation of well-resolved parts of a 0.25-nm electron-density map. The sequence of residues 343-346 could not be determined with chemical methods and was assigned from the size and shape of the amino acids in the electron-density map. An important tool in the analysis of the amino sequence of peptide CB1 was the proteinase Lys-C from Lysobacter enzymogenes, which preferentially cleaves at lysine residues.This publication has 35 references indexed in Scilit:
- Primary and Tertiary Structure Studies of p-Hydroxybenzoate Hydroxylase from Pseudomonas fluorescensEuropean Journal of Biochemistry, 2005
- The Amino-Acid Sequence of the Three Smallest CNBr Peptides from p-Hydroxybenzoate Hydroxylase from Pseudomonas fluorescensEuropean Journal of Biochemistry, 2005
- Primary structure of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescensBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
- The Primary Structure of the Coat Protein of Alfalfa Mosaic Virus Strain VRU. A Hypothesis on the Occurrence of Two Conformations in the Assembly of the Protein ShellEuropean Journal of Biochemistry, 1979
- A Study of p‐Hydroxybenzoate Hydroxylase from Pseudomonas fluorescensEuropean Journal of Biochemistry, 1979
- Crystal structure of p-hydroxybenzoate hydroxylaseJournal of Molecular Biology, 1979
- Direct microsequence analysis of polypeptides using an improved sequenator, a nonprotein carrier (Polybrene), and high pressure liquid chromatographyBiochemistry, 1978
- Electron microscopic studies on microcrystals of parahydroxybenzoate hydroxylase from Pseudomonas fluorescensFEBS Letters, 1976
- Use of parvalbumin as a protecting protein in the sequenator: An easy and efficient way for sequencing small amounts of peptidesFEBS Letters, 1976
- The primary structure of aspartate aminotransferase from pig heart muscle determined in part using a protease with specificity for lysineFEBS Letters, 1974