Establishment of a nonproductive herpes simplex virus infection in rabbit kidney cells
- 1 July 1975
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 12 (1), 128-133
- https://doi.org/10.1128/iai.12.1.128-133.1975
Abstract
A nonproductive infection of rabbit kidney cells was established with a type 2 strain of herpes simplex virus after incubation of the virus-infected cells at 41 C. Although infectious virus could not be recovered from cells disrupted by freeze-thawing or sonication after incubation at 41 C, spontaneous reactivation of virus growth occurred in 84% of the cultures after lag periods of variable length when cultures of viable cells were transferred to 37 C and incubated. Forty-one percent of the cultures had lag periods of 4 or more days, and 24% had lag periods of 7 or more days. The longest lag period was 45 days. A similar infection was established in WI-38 cells, but attempts to establish the infection in human kidney cells was not successful. This nonproductive infection is being used to study the effects of various physical and chemical agents on herpes simplex virus in a latent state.Keywords
This publication has 25 references indexed in Scilit:
- Herpes Simplex Virus Latency in Cultured Human Cells Following Treatment with Cytosine ArabinosideJournal of General Virology, 1972
- Herpesvirus Type 2 Isolated from Cervical Tumor Cells Grown in Tissue CultureScience, 1971
- The Differentiation of Herpes Simplex Virus Type 1 and Type 2 by Temperature MarkersJournal of General Virology, 1971
- Latent Herpes Simplex Virus in Spinal Ganglia of MiceScience, 1971
- Development and Localization of Virus-specific Antigens During the Multiplication of Herpes Simplex Virus in BHK 21 CellsJournal of General Virology, 1968
- Virus isolated from the brain of a patient with multiple sclerosisExperimental Neurology, 1964
- Persistence of Herpes Simplex Virus in HeLa CellsNature, 1960
- The effect of the temperature of incubation on the formation and release of herpes simplex virus in infected FL cellsVirology, 1959
- Counting actively metabolizing tissue cultured cellsExperimental Cell Research, 1957
- Effect of Previous Sensory Loss On The Appearance of Herpes SimplexJournal of Neurosurgery, 1953