Preferential neurotoxicity of pentobarbital on nerve and glial cells in culture
- 1 May 1977
- journal article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 33 (5), 627-629
- https://doi.org/10.1007/bf01946540
Abstract
The effect of pentobarbital was studied in a mixed population of nerve and glial cells dissociated from brains of 7-day chick embryos and maintained in culture. Pentobarbital-Na was added in various concentrations ranging from 5×10−5 M to 1×10−3 M. The neuronal density was monitored by counting of neurons, neuronal identity was established by staining for Nissl Bodies and acetylcholinesterase. Over a culture period of 3 weeks, it was found that the barbiturate exerts a preferential dose-dependent cytotoxic effect on neurons.Keywords
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