N‐Glycosylation of Yeast Proteins
- 1 May 1981
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 116 (1), 101-108
- https://doi.org/10.1111/j.1432-1033.1981.tb05306.x
Abstract
The enzyme transferring the oligosaccharide from DolPP‐(GlcNAc)2(Man)9(Glc)3 to asparagine residues of glycoproteins has been solubilized from yeast membranes by extraction with detergents. Enzyme activity was tested by measuring transfer of the glycosyl moiety from DolPP‐[14C]saccharidcs to the hexapeptide Tyr‐Asn‐Leu‐Thr‐Ser‐Val. The rate of transfer was linear for 20 min, with about 40% of dolichyl‐diphosphate‐bound radioactivity transferred to the peptide. The solubilized enzyme has been characterized as follows:This publication has 29 references indexed in Scilit:
- Dolichyl phosphate linked sugars as intermediates in the synthesis of yeast mannoproteins: an in vivo studyArchiv für Mikrobiologie, 1980
- Solubilization of oligosaccharide tranferase and glucosidase activities from thyroid rough microsomesFEBS Letters, 1980
- Microsomal glucosidases acting on the saccharide moiety of the glucose-containing dolichyl diphosphate oligosaccharideBiochemical and Biophysical Research Communications, 1979
- Studies on the acceptor specificity of asparagine‐N‐glycosyl‐transferase from rat liverFEBS Letters, 1979
- The role of lipid intermediates in the glycosylation of proteins in the eucaryotic cellBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1979
- Enzymatic N‐glycosylation of synthetic ASN‐X‐THR containing peptidesFEBS Letters, 1978
- Biosynthesis and characterization of large dolichyl diphosphate-linked oligosaccharides in Sacchromyces cerevisiaeBiochimica et Biophysica Acta (BBA) - General Subjects, 1978
- The specific site of tunicamycin inhibition in the formation of dolichol‐bound N‐acetylglucosamine derivativesFEBS Letters, 1976
- Identification of the Lipid Intermediate in Yeast Mannan BiosynthesisEuropean Journal of Biochemistry, 1973
- Heterogeneity of the carbohydrate moiety of crystalline ovalbuminBiochimica et Biophysica Acta, 1963