Delayed stimulatory effect of cyclic AMP on bone resorption in vitro
- 1 June 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 97 (2), 281-288
- https://doi.org/10.1530/acta.0.0970281
Abstract
The effect of dibutyryl cAMP (dbcAMP) and the phosphodiesterase inhibitors 3-isobutyl methylxanthine (IBMX) and theophylline on bone resorption was studied in an organ culture system for 96-144 h using half calvaria from 6-7 day old mice. The magnitude of resorption was assessed by measuring the release from the bones of previously incorporated 45Ca. dbcAMP, IBMX and theophylline, following a lag period or a period of reduced bone resorption, all progressively increased mineral mobilization. Although the continuous presence of dbcAMP increased mineral mobilization more than a temporary exposure, a limited treatment of 24 h with the nucleotide was sufficient to bring about the delayed stimulatory response. Apparently cAMP is not a mediator of the early stages of parathyroid hormone (PTH)- and prostaglandin E2 (PGE2)-stimulated bone resorption. The role played by cAMP may be related to the capacity of PTH and PGE2 to develop new osteoclasts, a phenomenon which takes more than 24 h to be observed.This publication has 5 references indexed in Scilit:
- Inhibition of bone resorption and lysosomal enzyme release from calvarial bones cultured for 24 hours: synergism between cyclic AMP analogues and phosphodiesterase inhibitorsActa Endocrinologica, 1980
- Time-related changes in the ultrastructure of osteoclasts after injection of parathyroid hormone in young ratsCalcified Tissue International, 1979
- Comparison of the effects of 1,25-dihydroxycholecalciferol, prostaglandin E2, and osteoclast-activating factor with parathyroid hormone on the ultrastructure of osteoclasts in cultured long bones of fetal ratsCalcified Tissue International, 1979
- INHIBITORY EFFECT OF DIBUTYRYL CYCLIC AMP ON THE RELEASE OF CALCIUM, INORGANIC PHOSPHATE AND LYSOSOMAL ENZYMES FROM CALVARIAL BONES CULTURED FOR 24 HOURSActa Endocrinologica, 1979
- Inhibition by cholera toxin of parathyroid hormone-induced calcium release from bone in cultureBiochemical and Biophysical Research Communications, 1977