Über das Absorptionsspektrum des Bilirubins in verschiedenen Lösungsmitteln. III. Mitteilung.

Abstract

A report of spectrum studies of bilirubin dissolved in chloroform, alcohol, and in normal serum, of colloidal bilirubin in water and of bilirubin present in two treated serums (designated as direct- and indirect-coupled, on the basis of the coupling of the bilirubin present with the Erlichschen diazo-solution). The absorption spectrum of bilirubin in human serum is similar to, though not exactly the same as, bilirubin dissolved in chloroform. The absorption curves of different diazotized serums vary in the short wave lengths of the spectrum, due to differences in content of uncoupled bilirubin. Bilirubin, from direct-and indirect-coupled serums, does not vary in spectrum properties or in crystalline form.