Simultaneous fluorescent retrograde axonal tracing and immunofluorescent characterization of neurons
- 1 January 1980
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 5 (6), 479-484
- https://doi.org/10.1002/jnr.490050603
Abstract
A simple method is described for simultaneously double labeling single neurons with fluorescent retrograde axonal tracers and immunofluorescence using an antibody to serotonin. Caudata-putamen injection of orange fluorescent propidium iodide, in particular, proved effective in retrogradely labeling dorsal raphe cell bodies. These same perikarya could be stained green with the indirect immunofluorescent method, using green fluorescent fluorescein isothiocynate (FITC) as the second antibody.Keywords
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