FLOCCULATION-SOME OBSERVATIONS ON THE SURFACE CHARGES OF YEAST CELLS
Open Access
- 4 March 1975
- journal article
- research article
- Published by The Institute of Brewing & Distilling in Journal of the Institute of Brewing
- Vol. 81 (2), 107-110
- https://doi.org/10.1002/j.2050-0416.1975.tb03670.x
Abstract
Surface charges on flocculent and non-flocculent yeast cells have been measured by micro-electrophoresis. Yeasts were grown both in calcium deficient and in complete medium and particular attention was paid to changes as cells passed from a logarithimic to a stationary phase of growth. Many of the ionogenic groups contributing to surface charge are situated well within the cell wall; charges on cells from the calcium-deficient medium were higher than on cells from the complete medium. A pH-dependent rearrangement of an underlying surface protein layer is postulated for the flocculent yeast Saccharomyces cerevisiae Strain NCYC 1109. No evidence of a similar rearrangement was found with other flocculent strains examined. The results are discussed in relation to ‘calcium bridge’ formation.This publication has 19 references indexed in Scilit:
- Charges on fungal sporesPesticide Science, 1973
- FLOCCULATION IN SACCHAROMYCES CEREVISIAE AS INFLUENCED BY WORT COMPOSITION AND BY ACTIDIONEJournal of the Institute of Brewing, 1972
- The Electrophoretic Properties and Some Surface Components of Penicillium ConidiaJournal of General Microbiology, 1970
- Evidence against the presence of fibres or chemically distinct layers in the cell wall ofSaccharomycesAntonie van Leeuwenhoek, 1970
- The Electrokinetic Properties of Some Fungal SporesJournal of General Microbiology, 1969
- Some physical investigations of the behaviour of bacterial surfaces XII. The effect of fimbriae on the electrophoretic mobility of some capsular and non-capsular bacteria of the Coli-Aerogenes groupBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1966
- The role of calcium in the increase in flocculence of yeast growing in the presence of copperProceedings of the Royal Society of London. B. Biological Sciences, 1965
- Some physical investigations of the behaviour of bacterial surfaces VI. Chemical modification of surface componentsBiochimica et Biophysica Acta, 1963
- The Influence of pH and Ionic Strength on the Electrokinetic Stability of the Human Erythrocyte MembraneThe Journal of general physiology, 1959
- The structure of the yeast cell wall - I. Identification of charged groups at the surfaceProceedings of the Royal Society of London. B. Biological Sciences, 1958