Requirement of RNA Polymerase III Transcription Factors for in Vitro Position-Specific Integration of a Retroviruslike Element
Open Access
- 10 March 1995
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 267 (5203), 1488-1491
- https://doi.org/10.1126/science.7878467
Abstract
The yeast retroviruslike element Ty3 inserts at the transcription initiation sites of genes transcribed by RNA polymerase III (Pol III). An in vitro integration assay was developed with the use of Ty3 viruslike particles and a modified SUP2 tyrosine transfer RNA (tRNA(Tyr)) gene target. Integration was position-specific and required Ty3 integrase, Pol III transcription factor (TF) IIIB-, TFIIIC-, and Pol III-containing fractions showed that TFIIIB and TFIIIC, together, were sufficient for position-specific Ty3 integration, but not for transcription. This report demonstrates that in vitro integration of a retroelement can be targeted by cellular proteins.Keywords
This publication has 39 references indexed in Scilit:
- Binding and Stimulation of HIV-1 Integrase by a Human Homolog of Yeast Transcription Factor SNF5Science, 1994
- Distribution of targets for avian retrovirus DNA integration in vivo.Genes & Development, 1994
- Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequencesCell, 1993
- Hotspots and warm spots: integration specificity of retroelementsTrends in Genetics, 1992
- Ty3 integrates within the region of RNA polymerase III transcription initiation.Genes & Development, 1992
- FLP Recombinase of the 2 μm circle plasmid of Saccharomyces cerevisiae bends its DNA targetJournal of Molecular Biology, 1989
- TRANSCRIPTION BY RNA POLYMERASE IIIAnnual Review of Biochemistry, 1988
- Structure of the DNA-Eco RI Endonuclease Recognition Complex at 3 Å ResolutionScience, 1986
- Preferential integration of yeast transposable element Ty into a promoter regionNature, 1984
- The promoter sequence of a yeast tRNAtyr geneCell, 1983