Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
Top Cited Papers
Open Access
- 4 August 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 38 (19), e181
- https://doi.org/10.1093/nar/gkq684
Abstract
The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.Keywords
This publication has 16 references indexed in Scilit:
- Dnmt1 and Dnmt3a maintain DNA methylation and regulate synaptic function in adult forebrain neuronsNature Neuroscience, 2010
- The Behaviour of 5-Hydroxymethylcytosine in Bisulfite SequencingPLOS ONE, 2010
- DNA methylation‐mediated epigenetic controlJournal of Cellular Biochemistry, 2009
- Oxygenase Catalyzed 5-Methylcytosine HydroxylationChemistry & Biology, 2009
- Prediction of novel families of enzymes involved in oxidative and other complex modifications of bases in nucleic acidsCell Cycle, 2009
- Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by MLL Partner TET1Science, 2009
- The Nuclear DNA Base 5-Hydroxymethylcytosine Is Present in Purkinje Neurons and the BrainScience, 2009
- Maintenance of self‐renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3bGenes to Cells, 2006
- TET1, a member of a novel protein family, is fused to MLL in acute myeloid leukemia containing the t(10;11)(q22;q23)Leukemia, 2003
- Reaction of bisulfite with the 5-hydroxymethyl group in pyrimidines and in phage DNAsBiochemistry, 1979