Development and Evaluation of a Broadly Reactive TaqMan Assay for Rapid Detection of Hepatitis A Virus
Open Access
- 1 June 2005
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 71 (6), 3359-3363
- https://doi.org/10.1128/aem.71.6.3359-3363.2005
Abstract
Primers and a TaqMan probe for the 5′-untranslated region (UTR) of the hepatitis A virus (HAV) genome were designed and evaluated. The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provides an important screening tool for rapid quantitative HAV detection in clinical or environmental samples.Keywords
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