Transformation in Restriction-deficient Salmonella typhimurium LT2
- 1 September 1989
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 135 (9), 2561-2567
- https://doi.org/10.1099/00221287-135-9-2561
Abstract
Stable restriction-deficient, modification-proficient galE (JR501) and F''galE+ (JR502) strains of Salmonella typhimurium were constructed and the effects of restriction on transformation by plasmid pBR322 were tested. Several factors which affect transformation efficiency were systematically examined to determine optimum transformation conditions and a simplified method is presented.This publication has 3 references indexed in Scilit:
- Positive selection for loss of tetracycline resistanceJournal of Bacteriology, 1980
- Deoxyribonucleic acid restriction and modification systems in Salmonella: chromosomally located systems of different serotypesJournal of Bacteriology, 1980
- SMOB MUTATION SUPPRESSING CELL FILAMENTATION AND ABILITY TO SUPPORT THE MULTIPLICATION OF PHAGE-P22 IN SALMONELLA-TYPHIMURIUM1979