Infection and Synthesis Rate of Southern Bean Mosaic Virus in Soybean Callus Cells under Selected Cultural Conditions

Abstract

Soybean (Glycine max ''Harosoy 63'') callus cells grown in either Eriksson''s or Linsmaier and Skoog''s media were inoculated with southern bean mosaic virus (SBMV) and then incubated in either liquid or agar media. The composition and type (liquid or agar) of the medium significantly affected the growth curve of the virus. Assays in Phaseolus vulgaris ''Pinto'' leaves indicated that soybean callus grown in liquid and incubated in liquid medium after inoculation gave the most rapid virus growth rate compared with that of ''Prince'' bean callus treated identically or with infected soybean plants grown in the greenhouse. Vortexing of the cell-suspension/virus-inoculum mixture was not necessary. Infection was achieved by adding virus inoculum to a suspension culture of callus cells, washing the cells with fresh medium and incubating the cells in liquid media on a rotary shaker at 120 rpm.