REGULATION OF ANTIBODY-RESPONSE IN DIFFERENT IMMUNOGLOBULIN CLASSES .1. SELECTIVE SUPPRESSION OF ANTI-DNP IGE ANTIBODY-RESPONSE BY PREADMINISTRATION OF DNP-COUPLED MYCOBACTERIUM

Abstract

Selective suppression of the [mouse] Ig[immunoglobulin]E antibody response was demonstrated. Preadministration of DNP[dinitrophenol]-coupled M. [tuberculosis] (DNP-Tcb) inhibited the formation of anti-DNP IgE antibody induced by DNP-OA [ovalbumin] without any suppressive effect on the anti-DNP IgG antibody response. Secondary anti-DNP IgE antibody response by DNP-OA was significantly depressed by the preadministration of DNP-Tbc. Anti-OA IgE antibody response induced by DNP-OA was depressed by DNP-Tbc, whereas anti-OA IgE antibody response induced by PAB[p-aminobenzoic acid]-OA was not affected by the preadministration of DNP-Tbc. Pre-immunization with DNP-MGG induced much higher anti-DNP IgG antibody response from DNP-Tbc, but DNP-MCG [mouse IgG] did not suppress the induction of anti-DNP IgE antibody. The transfer of DNP-Tbc-primed spleen cells into normal mice depressed the anti-DNP IgE antibody response. B [bone marrow derived] cell-depleted cell populations showed a comparable inhibitory effect to that of unfractionated DNP-Tbc primed cells. In the adoptive cell transfer experiment, DNP-Tbc-primed cells depressed anti-DNP IgE antibody response of DNP-OA-primed cells when these cells were stimualted with DNP-OA. DNP-specific T [thymus-derived] cells induced with DNP-Tbc selectively inhibited the IgE antibody response.