Validation of housekeeping genes for normalizing RNA expression in real-time PCR
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Open Access
- 1 July 2004
- journal article
- research article
- Published by Taylor & Francis in BioTechniques
- Vol. 37 (1), 112-119
- https://doi.org/10.2144/04371rr03
Abstract
Analysis of RNA expression using techniques like real-time PCR has traditionally used reference or housekeeping genes to control for error between samples. This practice is being questioned as it b... Normalizing RNA Expression Normalizing RNA levels to internal or housekeeping reference standards is widely used to control for sample error when measuring RNA expression levels. Choice of an appro...Keywords
This publication has 18 references indexed in Scilit:
- Expression of Th1-mediated immunity in mouse lungs induces aMycobacterium tuberculosistranscription pattern characteristic of nonreplicating persistenceProceedings of the National Academy of Sciences, 2002
- Dynamics of Gamma Interferon, Interleukin-12 (IL-12), IL-10, and Transforming Growth Factor β mRNA Expression in PrimaryMycobacterium bovisBCG Infection in Guinea Pigs Measured by a Real-Time Fluorogenic Reverse Transcription-PCR AssayInfection and Immunity, 2002
- beta-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levelsThorax, 2002
- Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problemsJournal of Molecular Endocrinology, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Identification and Validation of Endogenous Reference Genes for Expression Profiling of T Helper Cell Differentiation by Quantitative Real-Time RT-PCRAnalytical Biochemistry, 2001
- IL-4 Influences Apoptosis of Mycobacterium-Reactive Lymphocytes in the Presence of TNF-αThe Journal of Immunology, 2001
- Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysJournal of Molecular Endocrinology, 2000
- Type 2 Cytokine Gene Activation and Its Relationship to Extent of Disease in Patients with TuberculosisThe Journal of Infectious Diseases, 2000
- Restricted production of interleukin 4 by activated human T cells.Proceedings of the National Academy of Sciences, 1988