Nucleotide Sequence of the Simian Virus 40 HindII + III Restriction Fragment A (Second Part of the T Antigen Gene)

Abstract

The nucleotide sequence of the 2nd part of the SV 40 DNA HindII + III restriction fragment A is presented. The sequence extends from map position 0.533-0.424 and together with the 1st part of Hind-A completes the total Hind-A sequence, comprising 1169 base pairs. The 2nd half of Hind-A includes the region corresponding to the second splicing boundary common to small tumor antigen (small-t) and large tumor antigen (large-T) mRNA and it contains coding information for an internal portion of large-T antigen. Two similar secondary structures of reasonable thermodynamic stability can be proposed for the nucleotide sequence of the pre-mRNA corresponding to the region reported here. Their possible relevance to the splicing of the SV 40 early mRNA is discussed. The deduced amino acid sequence is 188 residues long and contains a Lys-Lys-Lys-Arg-Lys stretch which may be involved in the DNA binding capacity of large-T. A presumptive phosphorylation site is also present.