Notizen: Versuche zum Mechanismus der Pasteur-Reaktion. Der Einfluß von Phosphationen auf die Aktivität der strukturgebundenen Hexokinase

Abstract

Experiments with structurally bound mitochondrial hexokinase from rat brain show that the enzyme activity depends on the phosphate concentration. Glucose consumption, and the glucose-6-phosphate determined enzymatically at the end of incubation, increased by 40-60% at a phosphate concentration of 8 X 10-3 moles per liter in contrast to mixtures to which no phosphate was added. The conversion of other substrates, such as mannose and desoxyglycose, which form no phosphoric esters that inhibit hexokinase, in contrast, was not increased by phosphate.