A procedure for the purification of DNA from tobacco cell cultures and isolation of tobacco single-copy DNA
- 15 June 1978
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Botany
- Vol. 56 (12), 1453-1457
- https://doi.org/10.1139/b78-169
Abstract
A procedure is presented for the purification of DNA from tobacco callus cells. The procedure can be scaled up for the preparation in large quantity of DNA free of RNA, protein, and polysaccharide. The DNA prepared by this procedure has the same melting temperature and buoyant density as tobacco DNA prepared by previously published procedures and is of sufficient purity to be used for analysis of the reassociation kinetics of tobacco callus DNA. The DNA has been used as a source for the isolation of tobacco single-copy DNA.This publication has 8 references indexed in Scilit:
- Tables for estimating sedimentation through linear concentration gradients of sucrose solutionAnalytical Biochemistry, 1967
- Sedimentation studies of the size and shape of DNAJournal of Molecular Biology, 1965
- Organic Growth Factor Requirements of Tobacco Tissue CulturesPhysiologia Plantarum, 1965
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961
- Synthesis of ribonucleic acid in plantsExperimental Cell Research, 1959
- A new method for the isolation of ribonucleic acids from mammalian tissuesBiochemical Journal, 1956
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951