Blockage of AM V reverse transcriptase by antisense oligodeoxynucleotides
- 12 November 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 274 (1), 53-56
- https://doi.org/10.1016/0014-5793(90)81327-k
Abstract
Synthetic oligodeoxynucleotides, either unmodified or linked to an intercalating agent, have been used to prevent cDNA elongation by the AMV reverse transcriptase. Oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation of the RNA part bound to the antisense oligonucleotide by the RNase-H activity associated with the retroviral polymerase.Keywords
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