Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A‐acyl carrier protein transacylase of Escherichia coli

Abstract
We report the cloning and nucleotide sequence of the gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli, Malonyl transacylase has been overexpressed 155-fold compared to a wild-type strain, Overexpression of this enzyme alters the fatty acid composition of a wild-type E. coli strain; increased amounts of cis-vaccenate are incorporated into the membrane phospholipids.