Formation of Polymeric Glycoprotein Complexes from a Flavivirus: Tick-borne Encephalitis Virus
- 1 July 1980
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 49 (1), 125-132
- https://doi.org/10.1099/0022-1317-49-1-125
Abstract
Treatment of tick-borne encephalitis (TBE) virus with Triton X-100 (TX-100), octylglucoside (OG) or cetyltrimethylammonium bromide (CTAB) caused dissociation of the virus envelope into dimers or monomers of the glycoprotein V3. By centrifugation into detergent-free sucrose density gradients, these subunits reassociated and formed hemagglutinating homogeneous glycoprotein complexes sedimenting at 15-16, 16-18 and 11-12S after TX-100, OG and CTAB treatment, respectively. Glycoprotein complexes obtained after TX-100 solubilization contained less than 1% lipid and detergent by weight.This publication has 11 references indexed in Scilit:
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