Transcription of genes encoding enzymes involved in DNA synthesis during the cell cycle of Saccharomyces cerevisiae
- 1 September 1986
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 204 (3), 363-366
- https://doi.org/10.1007/bf00331011
Abstract
We have examined the pattern of transcription exhibited by four genes in the dTTP biosynthetic pathway of Saccharomyces cerevisiae. Consistent with the results reported previously by Storms et al. (1984), the TMP1 (or CDC21) gene encoding thymidylate synthase was found to be transcribed in a periodic manner during the cell cycle with maximal mRNA levels occurring just prior to the onset of DNA replication. Three other genes in this pathway DCD1, DUT1 and DFR1 encoding dCMP deaminase, dUTP pyrophosphatase and dihydrofolate reductase, respecitively, exhibited relatively constant levels of transcription throughout the cell cycle. These results, particularly for DFR1, are in marked contrast with those obtained in other eukaryotic systems which have suggested that, in general, genes encoding enzymes involved in DNA precursor synthesis are subject to cell cycle regulation. Thus, periodic transcription is not a property common to all genes involved in DNA replication in this eukaryote.This publication has 30 references indexed in Scilit:
- Tight coordination of ribonucleotide reduction and thymidylate synthesis in synchronous algaeFEBS Letters, 1983
- Cell-cycle regulation of yeast histone mRNACell, 1981
- Alterations in the levels of deoxyuridine triphosphatase, uracil-DNA glycosylase and AP endonuclease during the cell cycleExperimental Cell Research, 1980
- Ribonucleotide reductase activity in green algaeBiochemical and Biophysical Research Communications, 1976
- Ribonucleotide reductase activity during the cell cycle of Saccharomyces cerevisiaeArchives of Biochemistry and Biophysics, 1973
- Fluctuations in deoxycytidine monophosphate deaminase activity during the cell cycle in synchronous populations of HeLa cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Initiation of DNA Synthesis in a System of Synchronized L‐Cells: Rhythmicity of Thymidine Kinase ActivityEuropean Journal of Biochemistry, 1968
- Enzymic control of nucleic acid synthesis during synchronous growth of Chlorella pyrenoidosaBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- Enzymic control of nucleic acid synthesis during synchronous growth of Chlorella pyrenoidosaArchives of Biochemistry and Biophysics, 1966
- Variations in Phosphokinase Activities During the Cell Cycle in Synchronous Populations of HeLa CellsNature, 1965