Alcianblue/PAS or PAS/alcianblue?
- 1 March 1984
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 80 (2), 129-132
- https://doi.org/10.1007/bf00679985
Abstract
The combined alcian blue (AB)/PAS technic is widely used for the detection and characterization of mucosubstances in tissue sections. Mostly the sequence AB/PAS is used, occasionally also the reserved sequence PAS/AB. The present study shows clearly that the sequence of the combined technic, i.e. AB/PAS or PAS/AB is substantially influencing the results. So it could be demonstrated that by using the combination PAS/AB originally PAS-positive and AB-negative reacting mucosubstances become AB-posltive. This could be caused by periodic acid oxidation followed by addition of hydrogen sulfite to aldehyde group thus providing secondary basophilic resp. AB positive material.This publication has 10 references indexed in Scilit:
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