RESTRICTED LIGHT CHAIN SUBGROUP EXPRESSION ON HUMAN RHEUMATOID-FACTOR PARAPROTEINS DETERMINED BY MONOCLONAL-ANTIBODIES
- 1 November 1986
- journal article
- research article
- Vol. 59 (3), 473-478
Abstract
Two hybridoma antibodies specific for determinants on the VKIII light chain subgroup have been produced and characterized. Antibodies C7 and B12 reacted with distinct VKIII epitopes irrespective of association with heavy chain class or subclass. Epitopes recognized by C7 and B12 were expressed on the light chain of IgG, IgA, and IgM and Bence-Jones paraproteins from the VKIII subgroup. However, a preferential association of both epitopes with IgM RF paraproteins was demonstrated. Hybridomas C7 and B12 reacted with 12/12 and 10/12 IgM RF paraproteins, respectively, but only with 3/6 IgM paraproteins, with no RF activity. Both epitopes C7 and B12, were immunodominant and conformation dependent, being detected by HA, HAI and ELISA on intact light chain but not isolated VK.This publication has 20 references indexed in Scilit:
- Immunogenic and Antigenic Epitopes of Immunoglobulins X: Monoclonal Antibodies Specific for Human IgA, the IgA1 and IgA2 Subclasses and an nA2m(2) Iso‐Allotypic EpitopeVox Sanguinis, 1985
- A common idiotope on human rheumatoid factors identified by a hybridoma antibodyMolecular Immunology, 1983
- Immunogenic and antigenic epitopes of immunoglobulins—V. Reactivity of a panel of monoclonal antibodies with sub-fragments of human Fcγ and abnormal paraproteins having deletionsMolecular Immunology, 1983
- Variable region genes for the immunoglobulin framework are assembled from small segments of DNA—A hypothesisProceedings of the National Academy of Sciences, 1978
- Use of antibody-coated red cells for the sensitive detection of antigen and in rosette tests for cells bearing surface immunoglobulinsJournal of Immunological Methods, 1977
- Kappa Chain (VχIII) Subgroup‐Related Activity in an Idiotypic Anti‐Cold Agglutinin SerumScandinavian Journal of Immunology, 1976
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- Hypervariable Regions, Idiotypy, and the Antibody-Combining SiteAdvances in Immunology, 1975
- SIMILARITIES IN THE LIGHT CHAINS OF ANTI-γ-GLOBULINS SHOWING CROSS-IDIOTYPIC SPECIFICITIESThe Journal of Experimental Medicine, 1974
- Immunoglobulin M: Pentameric Fcμ Fragments Released by Trypsin at Higher TemperaturesProceedings of the National Academy of Sciences, 1970