Protein which interacts with a stimulatory factor of RNA polymerase II of Ehrlich ascites tumor cells

Abstract

When partially purified Ehrlich ascites [mouse mammary] tumor RNA polymerase II was further purified on a column of phosphocellulose, stimulation of its catalysis of RNA synthesis by stimulatory factor S-II was greatly decreased. This decrease in sensitivity to the stimulatory factor was reversible: the enzyme eluted from phosphocellulose became sensitive to the factor when mixed with a protein fraction eluted from the phosphocellulose at high salt concentration. Evidence was obtained that this protein, named helper protein, binds to the enzyme eluted from phosphocellulose, causing it to recover sensitivity to stimulatory factor S-II.