?In vitro? filbert (Corylus avellana L.) micropropagation from shoot and cotyledonary node segments

Abstract

Plantlets were successfully regenerated from shoot and cotyledonary node segments excised from 20 day old filbert seedlings. In both cases the optimum initiation and elongation of shoot buds was obtained after 15 days culture in 1/2K(h) medium plus BAP (25 μM) followed by 20 days culture in the same medium in presence of a reduced BAP concentration (0.5 or 2.5 μM). Maximum of functional roots were readily formed after 5 days of submersion of the basal end of shoots in 1/2K(h) liquid medium plus IBA (50 μM), then transferred to a fresh 1/2K(h) solid medium for a further 15 days. Following these two consecutive steps, root initiation and development was achieved in 80% of the explants. The histological origin of neoformed organs was studied.