Characterization of Allosteric Insulin Hexamers by Electrospray Ionization Mass Spectrometry
- 10 December 1998
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 71 (2), 384-387
- https://doi.org/10.1021/ac980753s
Abstract
Electrospray mass spectrometry is used at pH 8.0 in combination with accurate mass measurements to confirm the multiplicity of insulin in stable noncovalent complexes with Zn(II) ions. Determination of the nature and number of ligands involved in Zn(II) chelation is in agreement with crystal and solution structures. Counting the number of ligands participating in each center allows deduction of the geometric configuration of the ligand field and gives indirect information about the conformational state of the insulin monomers in solution.Keywords
This publication has 16 references indexed in Scilit:
- Gaseous Conformational Structures of Cytochrome cJournal of the American Chemical Society, 1998
- Characterization of the r-state insulin hexamer and its derivatives. The hexamer is stabilized by heterotropic ligand binding interactionsBiochemistry, 1991
- High performance sector mass spectrometers: Past and presentMass Spectrometry Reviews, 1989
- Comparison of solution structural flexibility and zinc binding domains for insulin, proinsulin, and miniproinsulinBiochemistry, 1989
- Phenol stabilizes more helix in a new symmetrical zinc insulin hexamerNature, 1989
- Fast atom bombardment and plasma desorption mass spectrometry of degraded insulins in intermediate and long‐acting preparationsRapid Communications in Mass Spectrometry, 1988
- High mass fragmentation. Matrix effects in plasma desorption mass spectrometryInternational Journal of Mass Spectrometry and Ion Processes, 1987
- 252Cf‐Plasma Desorption Mass SpectrometryMass Spectrometry Reviews, 1985
- Structure of insulin in 4-zinc insulinNature, 1976
- Excision of Thymine Dimers and Other Mismatched Sequences by DNA Polymerase of Escherichia coliNature, 1969