Chiral Separation of Reduced Haloperidol by Capillary Zone Electrophoresis with Heptakis (2,6-Di-o-Methyl)-β-cyclodextrin

Abstract

A simple capillary zone electrophoresis was developed for the chiral separation of reduced haloperidol, a chiral metabolite of antipsychotic drug, haloperidol. Reduced haloperidol was separated in a phosphate buffer (pH 2.5) using heptakis (2,6-di-O-methyl)-β-cyclodextrin as a chiral selector. Several parameters such as type of cyclodextrin, pH and concentration of buffer, applied voltage, concentration of chiral selector and detection wavelength that affected the chiral separation and detection of reduced haloperidol were discussed.