Thromboxane synthesis by sources other than platelets in association with complement-induced pulmonary leukostasis and pulmonary hypertension in sheep.
- 1 January 1983
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation Research
- Vol. 52 (1), 1-6
- https://doi.org/10.1161/01.res.52.1.1
Abstract
Infusion into sheep of plasma containing zymosan-activated complement produces leukopenia, pulmonary leukostasis, and pulmonary artery hypertension. We previously demonstrated a close relationship between the pulmonary vascular response and elevations of plasma thromboxane. We have investigated the source of thromboxane synthesis in this model. Plasma containing zymosan-activated complement added to whole blood did not stimulate thromboxane synthesis. This observation suggested that leukocytes do not synthesize thromboxane directly in response to complement added to whole blood did not stimulate thromboxane synthesis. This observation suggested that leukocytes do not synthesize thromboxane directly in response to complement. Sheep rendered severely thrombocytopenic by the administration of antiplatelet serum responded to complement infusion in the usual way. Pretreatment with aspirin (10 mg/kg) protected sheep against the pulmonary vascular response and completely blocked thromboxane synthesis. Transfusion of functional platelets did not restore these responses. Twenty-four hours after aspirin treatment, in vivo thromboxane synthesis was significantly greater than platelet thromboxane synthesis in vitro. Thromboxane is synthesized by a tissue which recovers cyclooxygenase enzyme activity at a rate that is more rapid than platelet turnover. Sheep lung synthesizes thromboxane actively in vitro. It is postulated that leukocytes exposed to activated complement components damage pulmonary vascular endothelial cells and stimulate synthesis of thromboxane A2 which causes pulmonary vasoconstriction.This publication has 15 references indexed in Scilit:
- Bovine endothelial cells in culture produce thromboxane as well as prostacyclin.JCI Insight, 1981
- Complement Activation during Cardiopulmonary BypassNew England Journal of Medicine, 1981
- Inhibition of Prostacyclin and Platelet Thromboxane A2after Low-Dose AspirinNew England Journal of Medicine, 1981
- Synthesis of thromboxane B2 and 6-keto-prostaglandin F1α by bovine gastric mucosal and muscle microsomesProstaglandins, 1980
- Oxygen radicals mediate endothelial cell damage by complement-stimulated granulocytes. An in vitro model of immune vascular damage.JCI Insight, 1978
- Complement activation and pulmonary leukotasis during nylon fiber filtration leukapheresisBlood, 1978
- Nylon fiber leukapheresis: associated complement component changes and cranulocytopeniaBlood, 1978
- NYLON FIBER LEUKAPHERESIS - ASSOCIATED COMPLEMENT COMPONENT CHANGES AND GRANULOCYTOPENIA1978
- COMPLEMENT ACTIVATION AND PULMONARY LEUKOSTASIS DURING NYLON FIBER FILTRATION LEUKAPHERESIS1978
- Complement and Leukocyte-Mediated Pulmonary Dysfunction in HemodialysisNew England Journal of Medicine, 1977