Antibody Formation in vitro by Separated Spleen Cells: Inhibition by Actinomycin or Chloramphenicol
- 30 October 1964
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 146 (3644), 659-661
- https://doi.org/10.1126/science.146.3644.659
Abstract
The formation of 19S antibody to poliovirus by rabbit spleen cells in vitro was interrupted by adding actinomycin D (1 to 10 micrograms per milliliter) for 30 minutes or longer. Shorter treatment (5 to 15 minutes) caused partial suppression. Antibody formation was slowly renewed upon removal of the drug. These results suggest that 19S antibody formation is contingent upon DNA-dependent RNA synthesis and that the genetic messenger has an average lifetime of one-half day or less. Treatment with chloramphenicol (20 to 50 micrograms per milliliter) for 3 days caused the cessation of 19S antibody formation.Keywords
This publication has 18 references indexed in Scilit:
- Actinomycin D: Its Effect on Antibody Formation in vitroScience, 1963
- Breakdown of Rat-Liver Ergosomes in vivo after Actinomycin Inhibition of Messenger RNA SynthesisScience, 1963
- Actinomycin D Inhibition of Deoxyribonucleic Acid-Dependent Synthesis of Ribonucleic AcidScience, 1962
- On the inhibition of RNA synthesis by actinomycinBiochemical and Biophysical Research Communications, 1962
- Effect of Actinomycin D on Cellular Nucleic Acid Synthesis and Virus ProductionScience, 1961
- The mode of action of actinomycin DBiochimica et Biophysica Acta, 1960
- Amino acid incorporation into protein by extracts of E. coliBiochimica et Biophysica Acta, 1960
- Zur Wirkungsweise von ActinomycinenHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1960
- DESTRUCTION OF SOME AGGLUTININS BUT NOT OF OTHERS BY TWO SULFHYDRYL COMPOUNDSActa Pathologica Microbiologica Scandinavica, 1958
- Dissociation of Human Serum MacroglobulinsScience, 1957