Blood barriers of the epididymis and vas deferens act asynchronously with the blood barrier of the testis in the mink (Mustela vison)

Abstract

The purpose of the present study was to determine whether the blood barrier of the epididymis and vas deferens acted synchronously or not with the blood barrier of the testis. The permeability of the blood-epididymis and blood-vas deferens barrier was tested in neonatal kit mink up to puberty and monthly in adult minks throughout the annual seasonal reproductive cycle. Attention was focused particularly on time intervals when the blood barrier of the testis has been documented to be permeable, namely, before puberty and during testicular regression in the adult. One of two electron-opaque permeability tracers was perfused into the blood stream: horseradish peroxidase (HRP) or lanthanum nitrate. The convoluted tube of the epididymis was divided into three anatomical regions: the caput, corpus, and cauda. The vas deferens was divided into proximal and distal regions. At birth and throughout puberty, the three regions of the epididymis and the two of the vas deferens showed a lumen and a competent blood barrier. In the adult, a lumen persisted in the epididymis and vas deferens throughout the annual seasonal reproductive cycle, and the blood barrier of the excurrent duct remained impermeable even when the blood barrier in the testis became momentarily permeable during testicular regression. When HRP was used to test the permeability of the blood-tissue barrier of the excurrent ducts, no tracer deposits were observed on the lumenal surface of the epithelium. Conversely, when lanthanum served as the tracer, deposits of the probe were associated with microvilli and intracellular membranes despite impermeability of tight junctions. The data show that the lanthanum technique can yield false-positive results. The findings also indicate that 1) a blood-excurrent duct barrier is established before the blood-testis barrier and 2) the two barriers act asynchronously. It is therefore plausible that they are modulated by distinct factors.