STUDIES ON CELL ENZYME SYSTEMS

Abstract

Solutions of the enzyme luciferase, extracted from Cypridina, were subjected at pH 6.8 to temperatures from 40–55°C. for times up to 24 hours. After the desired exposures samples were cooled rapidly to room temperature, mixed with luciferin, and the first order velocity constants (representing luciferase activity) of the resulting luminescent reactions were determined by a photo electric method.