Rapid Clinical Determination of Urinary 17-Ketosteroids
- 1 April 1943
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 3 (4), 200-205
- https://doi.org/10.1210/jcem-3-4-200
Abstract
The urine is hydrolyzed with 10% HC1, extracted for 10 mins. by refluxing with boiling CCl4, and the hormone content detd. by the alkaline m-dinitrobenzene technique. Tests of the accuracy of the method show a maximum variation of about [plus or minus] 5% for any stage of the process, and an overall variation of [plus or minus] 10% or less. Since the individual and daily variations are considerably in excess of this amt. the procedure is satisfactory for clinical use.This publication has 4 references indexed in Scilit:
- Urinary 17-Ketosteroid Assays in Clinical Medicine1Journal of Clinical Endocrinology & Metabolism, 1942
- The Daily Variation in 17-Ketosteroid Excretion of Men and WomenJournal of Clinical Endocrinology & Metabolism, 1941
- COLORIMETRIC ASSAY OF I7'KETOSTEROIDS IN URINE. A SURVEY OF THE USE OF THIS TEST IN ENDOCRINE INVESTIGATION, DIAGNOSIS AND THERAPY1Journal of Clinical Endocrinology & Metabolism, 1941
- EFFECT OF WATER ON ACCURACY OF M-DINITROBENZENE REACTION IN THE QUANTITATIVE ASSAY OF URINARY KETOSTEROIDS1Endocrinology, 1941