DIETARY REPAIR OF DEFECTIVE LIPOGENESIS AND CHOLESTEROGENESIS (FROM C14-ACETATE) IN THE LIVER OF THE HYPOPHYSECTOMIZED RAT1

Abstract

EARLIER reports from this laboratory dealt with the conversion of the C¹⁴ of acetate-1-C¹⁴ to cholesterol, fatty acids, and CO₂ by liver slices prepared from hypophysectomized rats that had been fed a stock diet adequate in all respects and containing ground whole wheat as the main carbohydrate source (Tomkins et al., 1952; Baruch and Chaikoff, 1955). Under those dietary conditions, the incorporation of the C¹⁴ into fatty acids and cholesterol was depressed, but that into CO₂ was well within the normal range. Since the availability of glucose is of importance in the maintenance of lipogenesis (Masoro et al., 1950; Lyon et al., 1952; Masri et al., 1952; Hirsch et al., 1954), we have investigated both lipogenesis and cholesterogenesis in the livers of hypophysectomized rats that were fed synthetic diets in which the amounts and type of carbohydrate were controlled.