A quantitative method for the separation of thyroid hormones and related compounds from serum and tissues with an anion-exchange resin
- 1 June 1959
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 72 (2), 310-313
- https://doi.org/10.1042/bj0720310
Abstract
The thyroid hormones and related compounds can be separated from biological material by absorption on the ion-exchange resin Dowex-1 equilibrated with acetic acid-sodium acetate buffer at pH 5.6. The iodinated compounds are quantitatively eluted when the pH is reduced with acetic acid solutions of increasing concentration. Thyroglobulin, monoiodotyrosine and diodotyrosine are eluted at pH 3.6, 3.0 and 2.2 respectively and thyroxine and triiodothyronine are eluted at pH 1.4. Iodide remains on the resin throughout. Dowex-1 can therefore be used to concentrate the thyroid hormones in biological material for chromatographie analysis.Keywords
This publication has 5 references indexed in Scilit:
- [Qualitative and quantitative separation of iodinated thyroidal amino acids by chromatography on anion exchanges resins (dowex)].1958
- [Study of thyroid function in the rat using radioiodine & anion exchange resins].1957
- THE PURIFICATION OF THYROXINE-BINDING PROTEIN BY IONEXCHANGE RESINS1Endocrinology, 1957
- Isolation and quantitative estimation of iodide, thyroxine, and substances related to thyroxine by means of an anion exchange resinArchives of Biochemistry and Biophysics, 1955
- THE PRESENCE OF FREE IODINATED COMPOUNDS IN THE THYROID AND THEIR PASSAGE INTO THE CIRCULATIONEndocrinology, 1951