Carrier Protein-mediated Transport of Neutral Amino Acids into Mung Bean Mitochondria

Abstract

The transport of neutral amino acids into mitochondria isolated from the hypocotyl of mung bean was studied by the swelling technique. Isolated mitochondria swelled when added to an isosmotic solution of proline, serine, methionine, threonine, alanine and glycine. The swelling was stereospecific in that it was faster in the L-amino acid than in the corresponding D-amino acid. Preincubation of the mitochondria with the sulfhydryl modifying reagents, p-mercuribenzoate and mersalyl, resulted in an inhibition of the swelling caused by proline, serine, threonine and glycine. The swelling induced by alanine was inhibited only by mersalyl, whereas that by methionine was inhibited only by p-mercuribenzoate. In all cases, the inhibition caused by the sulfhydryl modifying reagents was readily reversible by the subsequent treatment of the mitochondria with dithiothreitol. N-Ethylmaleimide, another sulfhydryl-modifying reagent, did not cause any inhibition of the swelling. The findings indicate the existence of a protein mediated mechanism for the transport of neutral amino acids into plant mitochondria.